Neuroplasticity Mediated by Altered Gene Expression

Altered gene expression of angiogenic factors induced by calcium-mediated dissociation of retinal pigment epithelial cells.

PURPOSE To examine the effect of loss of cell-cell contacts on the gene expression of vascular endothelial growth factor (VEGF) and other factors in primary culture of human retinal pigment epithelial (RPE) cells with real-time reverse transcription-PCR. METHODS The dissociation of postconfluent RPE cells was induced by calcium chelation, low-calcium medium, anti-E-cadherin, and anti-N-cadher...

Antihyperglycemic effect of Rosa damascena is mediated by PPAR.γ gene expression in animal model of insulin resistance

Insulin resistance is a condition in which insulin signaling and action are impaired in insulin sensitive tissues and results in hyperglycemia, hyperlipidemia and type 2 diabetes mellitus. Our previous studies have shown that rosa damascena has antihyperglycemic effects on diabetic and normal rats. Therefore, we conducted a study to evaluate the effect of this medicinal plant on insulin sensiti...

Antihyperglycemic effect of Rosa damascena is mediated by PPAR.γ gene expression in animal model of insulin resistance

Insulin resistance is a condition in which insulin signaling and action are impaired in insulin sensitive tissues and results in hyperglycemia, hyperlipidemia and type 2 diabetes mellitus. Our previous studies have shown that rosa damascena has antihyperglycemic effects on diabetic and normal rats. Therefore, we conducted a study to evaluate the effect of this medicinal plant on insulin sensiti...

Lentivirus-mediated Gene Expression

Altered gene expression in cerebral ischemia.

BACKGROUND Using the techniques of molecular biology, recent experimental studies have shown that cerebral ischemia induces a variety of changes in gene expression in the brain. SUMMARY OF REVIEW During the early postischemic stages, protein synthesis in the brain is generally suppressed, but specific genes are expressed and their corresponding proteins may be synthesized, such as immediate-e...